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林业科学 ›› 2006, Vol. 42 ›› Issue (2): 21-26.doi: 10.11707/j.1001-7488.20060204

• 论文及研究报告 • 上一篇    下一篇

铜锌对华北落叶松多酚氧化酶活性的影响

杨云龙 姚延梼 李俊英   

  1. 山西农业大学林学院,太谷030801
  • 收稿日期:2004-05-08 修回日期:1900-01-01 出版日期:2006-02-25 发布日期:2006-02-25

Effect of Copper and Zinc on Activity of Polyphenol Oxidase in Larix principis-rupprechtii

Yang Yunlong,Yao Yantao,Li Junying   

  1. Forestry college, Shanxi Agricultural University Taigu 030801
  • Received:2004-05-08 Revised:1900-01-01 Online:2006-02-25 Published:2006-02-25

摘要:

以华北落叶松3年生幼苗为试验材料,对不同生育期4种处理(对照、锌、铜、铜+锌)下各器官(叶、枝、干、主根、侧根)多酚氧化酶(PPO)活性进行测定分析,结果表明:华北落叶松幼苗各器官内多酚氧化酶活性差异极为显著,干中PPO活性在3月达225.2U·g-1,而叶中PPO活性在5月仅为57.3U·g-1。叶子中PPO活性呈递增变化趋势,10月最高,枝、干、主根、侧根的PPO活性在年循环周期内呈规律性变化,7月均达高峰期。铜、锌对各器官内PPO活性有明显的影响,而且与生育期有关。铜对PPO活性的增强作用达显著水平,尤以枝最为突出,10月份铜处理后枝中PPO活性比对照提高95.1%;锌对主根PPO活性的提高也有较明显影响,对其他器官PPO活性有不同程度的抑制作用。10月份锌处理后干中PPO活性比对照下降35.5%;铜、锌互作对干和主根PPO活性的提高表现出较大的协同作用。华北落叶松幼苗体内铜元素含量与PPO活性呈显著的正相关,铜、锌元素通过影响PPO活性而影响幼苗的生长及抗寒性。

关键词: 华北落叶松, 多酚氧化酶(PPO), 铜,

Abstract:

In order to study the effects of copper and zinc on polyphenol oxidase (PPO) activity, the PPO activity of 3-year-old Larix principis-rupprechtii seedlings under four treatments (control, zinc, copper, copper + zinc) in different growth periods was analysed. Five organs were leaf, branch, trunk, main root and lateral root. The results showed that the PPO activity patterns of various organs were quite different, the highest could reach 225.2 U·g-1 in trunk (March) and the lowest was 57.3 U·g-1 in leaves (May). PPO activity in leaves increased during growth season and reached the maximum in October, PPO activity in branch, trunk, main and lateral root had an orderly change, which was higher in July than in other time. Copper andor zinc affected significantly the PPO activity of various organs, which was closely related to growth periods. Copper could improve the PPO activity in different organs, especially in branch, and copper treatment increased by 95.1% compared with the control in October. Zinc made the PPO activity increase in main root and decrease in other organs, and zinc treatment decreased by 35.5% compared with the control in trunk (October). Copper and zinc were cooperative to improve the PPO activity in trunk and main root. There was significant positive correlation between copper content and PPO activity in L. principis-rupprechtii. Copper and zinc had effects on PPO activity, so as to affect the resistant ability of cold and the growth of seedlings.

Key words: Larix principis-rupprechtii, polyphenol oxidase (PPO), copper, zinc