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林业科学 ›› 2003, Vol. 39 ›› Issue (2): 168-170.doi: 10.11707/j.1001-7488.20030229

• 研究简报 • 上一篇    下一篇

胡杨盐诱导基因与盐抑制基因的差减杂交显示研究

白根本 沈昕 王沙生   

  1. 北京中医药大学,北京100029;北京林业大学,北京100083
  • 收稿日期:2000-05-04 修回日期:1900-01-01 出版日期:2003-03-25 发布日期:2003-03-25

DIFFERENTIAL EXPRESSION OF SALT-INDUCED/REPRESSED GENES IN POPULUS EUPHRATICA USING SUBTRACTIVE HYBRIDIZATION TECHNIQUE

Bai Genben,Shen Xin,Wang Shashen   

  1. Beijing University of Chinese Medicine Beijing100029;Beijing Forestry University Beijing100083
  • Received:2000-05-04 Revised:1900-01-01 Online:2003-03-25 Published:2003-03-25

关键词: 胡杨, 差减杂交, 基因筛选

Abstract:

Salt-induced/repressed genes were isolated at the transciption level in P.euphratica using subtractive hybridization technique.About 40 cDNA fragments were obtained with polyacrylamide gel electrophoresis from +6/-6 to +8/-8 cDNA hybridization cycles,which will be used for screen and cloning salt-tolerant genes of P.eupharatica and studying the mechanism of salt-tolerant.

Key words: P.euphratica, Subtractive hybridization, Gene screening